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This inconsistency in regulated species lists across the shared waters of the Great Lakes undermines the collective prevention efforts of the region, resulting in a “weakest link” problem, wherein the success of AIS management efforts is limited to the Davidson et al. [...] In the absence of a comprehensive data set on all species in the live trade, we developed a candidate list of potential regulated species by combining the list of all regulated species that met the inclusion criteria above and a list of unregulated species that had been identified as potential future invaders. [...] announcement of a list of “least wanted” species in 2012 (GSGP 2013) only 5 of these high-risk species were regulated in at least half of all jurisdictions, but by 2020, 19 of the 21 species were regulated across the majority of jurisdictions. [...] Number of plant species regulated by jurisdiction, as of the start of 2008, 2014 and 2020, with the total number of unique species regulated in one or more jurisdiction (69) and the number of regulated “least wanted” species (out of 7). [...] Number of animal species regulated by jurisdiction, as of the start of 2008, 2014 and 2020 with the total number of unique species regulated in one or more jurisdiction (67) and the number of regulated “least wanted” species (out of 14).