Effects of diatom aldehydes on copepod reproduction: use of giant decadienal encapsulataed- liposomes

Effects of diatom aldehydes on copepod reproduction: use of giant decadienal encapsulataed- liposomes Isabella Buttino Effects of diatom aldehydes on copepod reproduction: use of giant liposomes encapsulated with decadienal Stazione Zoologica “Anton Dohrn” Napoli (Italy) and Dept. [...] costatum V IV DD 1.5 µg/ml III (7.3 x10 4 cells/ml) IVIII II II I I VI VIV V IV IV III III II II Day 3 I D ay 3 I 0 4 8 12 16 20 24 28 32 36 0 4 8 12 16 20 24 28 32 36 1 0 0 1 0 0 Estimated female Estimated female 8 0 ingestion 4.2 pg DD/hour 8 0 ingestion 30 pg DD/hour (IR= 1000 cells/h) 6 0 (Considering 30 fg DD/cell) 6 0 4 0 4 0 2 0 2 0 D a y 3 0 0 0 4 8 1 2 1 6 2 0 2 4 2 8 3 2 3 6 0 4 8 1 2 1. [...] Long-term effects of liposome ingestion at different lipid concentrations Pro Lipo A (46 µg/ml) Pro + Lipo B (5.5 µg/ml) 100 Lipo C (3.1 µg/ml) Lipo D (1.5 µg/ml) 125 75 100 50 75 25 50 25 0 0 1 2 3 4 5 6 7 8 910 0 Days 0 1 2 3 4 5 6 7 8 9 10 Days Buttino et al., in prep. [...] % survival fecal pellets couple -1 Effects of decadienal-encapsulating liposomes on copepod egg viability Final concentration of DD in liposomes Final concentration of DD in liposomes 3 ± 0.23 ng/ml 4.6 ± 1.2 ng/ml Pro+Lipo Decadienal Pro Calanus helgolandicus Temora stylifera Pro+Lipo blank 100 100 75 75 50 50 25 25 0 0 0 1 2 3 4 5 6 7 8 9 10 0 1 2 3 4 5 6 7 8 9 10 Days Days Corresponding to 0.42. [...] % Hatching success % Hatching success Effects of decadienal-encapsulating liposomes on female survival Temora stylifera ProPro+LipoDD Calanus helgolandicus Pro+Lipoblank 100 100 75 75 50 50 25 25 0 0 1 2 3 4 5 6 7 8 9 10 00 2 4 6 8 10 Days Days Buttino et al., in prep.